LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1685..1781) /label=attL2 /note="attL2 (c)" misc_signal complement(1796..1815) /note="T7 Promoter/priming site (c)" primer_bind 1823..1839 /note="M13 Reverse priming site" gene 1952..2761 /note="Kanamycin resistance gene" rep_origin 2882..3555 /note="pUC origin" vector join(1693..3558,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1678 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17148 IMAGE:4158309" /tissue_type="Brain, anaplastic oligodendroglioma with 1p/19q loss" /clone_lib="NCI_CGAP_Brn67" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1678 /gene="CHST10" /gene_synonym=HNK-1ST /gene_synonym=HNK1ST /gene_synonym=MGC17148 /db_xref="GeneID:9486" /db_xref="HGNC:19650" /db_xref="MIM:606376" CDS 692..1678 /dnas_title="carbohydrate sulfotransferase 10" /gene="CHST10" /gene_synonym=HNK-1ST /gene_synonym=HNK1ST /gene_synonym=MGC17148 /codon_start=1 /product="carbohydrate sulfotransferase 10" /protein_id="AAH10441.1" /db_xref="GI:14714607" /db_xref="GeneID:9486" /db_xref="HGNC:19650" /db_xref="MIM:606376" /translation="MHHQWLLLAACFWVIFMFMVASKFITLTFKDPDVYSAKQEFLFL TTMPEVRKLPEEKHIPEELKPTGKELPDSQLVQPLVYMERLELIRNVCRDDALKNLSH TPVSKFVLDRIFVCDKHKILFCQTPKVGNTQWKKVLIVLNGAFSSIEEIPENVVHDHE KNGLPRLSSFSDAEIQKRLKTYFKFFIVRDPFERLISAFKDKFVHNPRFEPWYRHEIA PGIIRKYRRNRTETRGIQFEDFVRYLGDPNHRWLDLQFGDHIIHWVTYVELCAPCEIM YSVIGHHETLEDDAPYILKEAGIDHLVSYPTIPPGITVYNRTKVEHYFLGISKRDIRR LYARFEGDFKLFGYQKPDFLLN" misc_feature 692..1678 /note="CHST10 coding region" misc_difference 1306..1306 /gene="CHST10" /gene_synonym=HNK-1ST /gene_synonym=HNK1ST /gene_synonym=MGC17148 /note="'G' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cacctttaaa gacccagatg tgtacagtgc 721 caaacaggag tttctgttcc tgacaaccat gccggaagtg aggaagttgc cagaagagaa 781 gcacattcct gaggaactga agccaactgg gaaggagctt ccagacagcc agctcgttca 841 gcccctggtc tacatggagc gcctggaact catcagaaac gtctgcaggg atgatgccct 901 gaagaatctc tcgcacactc ctgtctccaa gtttgtcctg gaccgaatat ttgtctgtga 961 caagcacaag attcttttct gccagactcc caaagtgggc aacacccagt ggaagaaagt 1021 gctgattgtt ctaaatggag cattttcttc cattgaggag atccccgaaa acgtggtgca 1081 cgaccacgag aagaacggcc ttcctcggct ctcttccttc agtgatgcag aaattcagaa 1141 gcgattgaaa acatacttca agttttttat tgtaagagat cccttcgaaa gacttatttc 1201 tgcatttaag gataaatttg ttcacaatcc ccggtttgag ccttggtaca ggcatgagat 1261 tgctcctggc atcatcagaa aatacaggag gaaccggaca gagacgcggg ggatccagtt 1321 tgaagatttc gtgcgctacc tcggcgatcc gaaccacaga tggctagacc ttcagtttgg 1381 ggaccacatc attcactggg tgacgtatgt agagctctgt gctccctgtg agataatgta 1441 cagtgtgatt ggacaccacg agaccctgga ggacgatgcc ccatacatct taaaagaggc 1501 tggcattgac cacctggtgt catacccgac tatccctccg ggcattaccg tgtataacag 1561 aaccaaggtg gagcactatt tcctgggcat cagcaaacga gacatccgac gcctgtatgc 1621 ccgtttcgaa ggggacttta agctctttgg gtaccagaaa ccagactttt tgctaaacTA 1681 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 1741 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 1801 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 1861 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 1921 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 1981 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2041 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2101 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2161 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2221 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2281 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2341 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2401 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2461 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 2521 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 2581 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 2641 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 2701 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 2761 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 2821 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 2881 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 2941 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3001 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3061 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3121 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3181 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3241 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3301 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3361 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3421 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3481 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 3541 GCCTTTTGCT CACATGTT //